WB analysis of mouse cardiac myocyte lysate using GTX22478 Merlin (phospho Ser518) antibody.
Lane 1 : Mock
Lane 2 : 4 nM CLA-treated cells
Lane 3 : 20 nM CLA-treated cells
Lane 4 : 100 nM CLA-treated cells
Dilution : 1:1000

Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 (GTX22478) was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amersham's substrate system. Other detection methods will yield similar results.