GeneTex
United States (US)

Merlin (phospho Ser518) antibody

GTX32340_20190131_IHC-P_19013102_585.jpg
GTX32340_20190131_WB_19013102_259.jpg

Cat No. GTX32340

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

70 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of Merlin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neurofibromin 2,Acn,Banf,Sch,Nf2

Cellular Localization

Cell projection,Cytoplasm,Cytoplasmic granule,Cytoskeleton,Filopodium membrane,Nucleus,Perinuclear region,Ruffle membrane

Background

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32340_20190131_IHC-P_19013102_585.jpg

GTX32340 IHC-P Image

IHC-P analysis of formalin fixed human muscle tissue section using GTX32340 Merlin (phospho Ser518) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32340_20190131_WB_19013102_259.jpg

GTX32340 WB Image

WB analysis of IFN-treated MCF7 (A), IFN-treated Jurkat (B) whole cell lysates using GTX32340 Merlin (phospho Ser518) antibody.