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Merlin (phospho Ser518) antibody

Anti-Merlin (phospho Ser518) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50291
Anti-Merlin (phospho Ser518) antibody used in Western Blot (WB). GTX50291
Anti-Merlin (phospho Ser518) antibody used in Western Blot (WB). GTX50291

Cat No. GTX50291

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 518 (R-L-S(p)-M-E) derived from human Merlin.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

neurofibromin 2 , ACN , BANF , SCH

Cellular Localization

Nucleus,Cytoplasm, cytoskeleton

Background

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Merlin (phospho Ser518) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50291

GTX50291 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50291 Merlin (phospho Ser518) antibody.

Anti-Merlin (phospho Ser518) antibody used in Western Blot (WB). GTX50291

GTX50291 WB Image

WB analysis of extracts from C6 cells treated with calf intestinal phosphatase (CIP) using GTX50291 Merlin (phospho Ser518) antibody.

Anti-Merlin (phospho Ser518) antibody used in Western Blot (WB). GTX50291

GTX50291 WB Image

WB analysis of extracts from HUVEC cells untreated (lane 1) or treated with IFN-a (lane 2) using GTX50291 Merlin (phospho Ser518) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339