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Merlin (phospho Ser518) antibody

Anti-Merlin (phospho Ser518) antibody used in Western Blot (WB). GTX79013

Cat No. GTX79013

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Merlin around the phosphorylation site of serine 518 (R-L-Sp-M-E).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11174630

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ACN,BANF,NF2,SCH,neurofibromin 2,Merlin,Neurofibromin 2b

Cellular Localization

Cell projection,Filopodium membrane,Ruffle membrane,Nucleus,Cytoplasm,Perinuclear region,Cytoplasmic granule,Cytoskeleton

Background

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Merlin (phospho Ser518) antibody used in Western Blot (WB). GTX79013

GTX79013 WB Image

WB analysis of HUVEC cells untreated or treated with IFN-α (100ng/ml, 15min) lysates using GTX79013 Merlin (phospho Ser518) antibody (Lane 3 and 4) and Merlin antibody (Lane 1 and 2).

REFERENCE

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REVIEW

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Package List Price ($)
$ 339