GeneTex
United States (US)

Mesothelin antibody [N1C2]

GTX105471_IHC_19011506_467.jpg
GTX105471_WB_19011506_711.jpg

Cat No. GTX105471

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

293T
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Mesothelin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MPF antibody, SMRP antibody, MSLN antibody, soluble MPF mesothelin related protein antibody, megakaryocyte potentiating factor antibody, pre-pro-megakaryocyte-potentiating factor antibody, mesothelin antibody, CAK1 antigen antibody

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor , Golgi apparatus , Megakaryocyte-potentiating factor: Secreted , Isoform 3: Secreted

Background

An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX105471_IHC_19011506_467.jpg

GTX105471 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer, using MSLN(GTX105471) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105471_WB_19011506_711.jpg

GTX105471 WB Image

Sample(30 µg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX105471 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf