GeneTex
  • Country / Location Selection

United States (US)

Mesothelin antibody [N1C2]

Anti-Mesothelin antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105471
Anti-Mesothelin antibody [N1C2] used in Western Blot (WB). GTX105471

Cat No. GTX105471

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

293T
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Mesothelin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

mesothelin , MPF , SMRP

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor , Golgi apparatus , Megakaryocyte-potentiating factor: Secreted , Isoform 3: Secreted

Background

An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Mesothelin antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105471

GTX105471 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer, using MSLN(GTX105471) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Mesothelin antibody [N1C2] used in Western Blot (WB). GTX105471

GTX105471 WB Image

Sample(30 µg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX105471 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
There are currently no references for Mesothelin antibody [N1C2] (GTX105471). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Mesothelin antibody [N1C2] (GTX105471). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169