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Midkine antibody

Cat No. GTX12739

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
50 μg ($289)

APPLICATION

Application Note

For ELISA: Use at a concentration of 0.5 μg/ml (100 μl/well) used to detect 0.2 ng/well recombinant Midkine. Biotin Conjugate use at a concentration of 0.2-0.3 μg/ml. For WB: Use at a concentration of 0.2 μg/ml used to detect 1.5 ng/lane of human recombinant Midkine under reducing and non-reducing conditions. Optimal dilutions/concentrations should be determined by the researcher.

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline pH 7.4 containing 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Midkine exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Its expression is predominant only for a short period from approximately one-half to two-thirds of the way through gestation; before and after that, it is barely detectable. Midkine was first found in differentiating mouse teratocarcinoma cells. It has neurotrophic activities and is mitogenic to certain, but not to all, fibroblast cell lines.

Research Area

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf