GeneTex
United States (US)

Midline-1 antibody

Anti-Midline-1 antibody used in Western Blot (WB). GTX34076
Anti-Midline-1 antibody used in Western Blot (WB). GTX34076

Cat No. GTX34076

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Specificity/Sensitivity

Midline-1 Polyclonal Antibody detects endogenous levels of Midline-1 protein.
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthesized peptide derived from the N-terminal region of human Midline-1 at AA range: 40-120.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Midline 1,Bbbg1,Fxy,Gbbb1,Midin,Ogs1,Os,Osx,Rnf59,Trim18,Xprf,Znfxy,Mid1

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Multiple different transcript variants are generated by alternate splicing; however, the full-length nature of some of the variants has not been determined. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES
Anti-Midline-1 antibody used in Western Blot (WB). GTX34076

GTX34076 WB Image

Western Blot (WB) analysis of specific cells using Midline-1 Polyclonal Antibody.

Anti-Midline-1 antibody used in Western Blot (WB). GTX34076

GTX34076 WB Image

Western Blot (WB) analysis of 293 cells using Midline-1 Polyclonal Antibody.

Package List Price ($)
$ 319