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Mig-2 antibody [14A11]

Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506
Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506
Anti-Mig-2 antibody [14A11] used in IHC (Paraffin sections) (IHC-P). GTX84506
Anti-Mig-2 antibody [14A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84506
Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506
Anti-Mig-2 antibody [14A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84506
Anti-Mig-2 antibody [14A11] used in Immunoprecipitation (IP). GTX84506
Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506

Cat No. GTX84506

Host Mouse
Clonality Monoclonal
Clone Name 14A11
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($379)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:50-1:100
IHC-P 1:50
IP 2ug/500ul
Not tested in other applications.

Calculated MW

78 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fermitin Family Member 2,Kind2,Mig2,Plekhc1,Unc112,Unc112B,Mig-2,Fermt2

Cellular Localization

Cytoplasm , cell cortex , cytoskeleton , Cell junction , focal adhesion

Background

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.

Database

Research Area

DATA IMAGES
Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506

GTX84506 WB Image

WB analysis of wild type and Mig-2 knock out HeLa cell lysate(10μg per lane) using GTX84506 Mig-2 antibody [14A11].
Dilution : 1:500

Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506

GTX84506 WB Image

WB analysis of various samples using GTX84506 Mig-2 antibody [14A11].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Mig-2 antibody [14A11] used in IHC (Paraffin sections) (IHC-P). GTX84506

GTX84506 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84506 Mig-2 antibody [14A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-Mig-2 antibody [14A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84506

GTX84506 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84506 Mig-2 antibody [14A11].

Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506

GTX84506 WB Image

WB analysis of various cell lines using GTX84506 Mig-2 antibody [14A11].
Loading : 35 ug per lane

Anti-Mig-2 antibody [14A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84506

GTX84506 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Mig-2 plasmid using GTX84506 Mig-2 antibody [14A11].

Anti-Mig-2 antibody [14A11] used in Immunoprecipitation (IP). GTX84506

GTX84506 IP Image

IP analysis of DDDDK tagged Mig-2 overexpressed HEK293T lysate using GTX84506 Mig-2 antibody [14A11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506

GTX84506 WB Image

WB analysis of HEK293T cells transfected with Mig-2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84506 Mig-2 antibody [14A11].
Loading : 5 ug per lane

REFERENCE
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REVIEW
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Package List Price ($)
$ 379