GeneTex
United States (US)

Mig-2 antibody [9E4]

KO/KD Overexpression
GTX84507_3371_IP_18121411_849.jpg
GTX84507_4431_WB_18121411_171.jpg
GTX84507_4430_20181228_WB_19011519_191.jpg

Cat No. GTX84507

Reference 2
Host Mouse
Clonality Monoclonal
Clone Name 9E4
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Rat, Dog, Monkey
See all Mig-2 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:50
IHC-P 1:150
IP 2ug/500ul
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Specificity/Sensitivity

GTX84507 is specific for human Mig-2.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686G11125 antibody, FLJ34213 antibody, FLJ44462 antibody, KIND2 antibody, MIG2 antibody, PLEKHC1 antibody, UNC112 antibody, UNC112B antibody, mig-2 antibody, FERMT2 antibody, "pleckstrin homology domain containing, family C (with FERM domain) member 1 antibody", kindlin-2 antibody, mitogen inducible gene 2 protein antibody, pleckstrin homology domain-containing family C member 1 antibody, kindlin 2 antibody, fermitin family homolog 2 antibody, "pleckstrin homology domain containing, family C member 1 antibody", mitogen-inducible gene 2 protein antibody, PH domain-containing family C member 1 antibody, fermitin family member 2 antibody

Cellular Localization

Cytoplasm , cell cortex , cytoskeleton , Cell junction , focal adhesion

Background

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.

Database

Research Area

DATA IMAGES
GTX84507_3371_IP_18121411_849.jpg

GTX84507 IP Image

IP analysis of DDDDK tagged Mig-2 overexpressed HEK293T lysate using GTX84507 Mig-2 antibody [9E4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate
GTX84507_4431_WB_18121411_171.jpg

GTX84507 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Mig-2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84507 Mig-2 antibody [9E4].
Loading : 5 ug per lane
GTX84507_4430_20181228_WB_19011519_191.jpg

GTX84507 WB Image

KO/KD

WB analysis of wild type and Mig-2 knock out HeLa cell lysate(10μg per lane) using GTX84507 Mig-2 antibody [9E4].
Dilution : 1:1000
REFERENCE

Application Reference

Sullivan SE et al. Hum Mol Genet 2018; (Epub ahead of print) Candidate-based screening via gene modulation in human neurons and astrocytes implicates FERMT2 in Aβ and TAU proteostasis.
Application : WB
Reactivity : Human
Chapuis J et al. Acta Neuropathol 2016; Genome-wide, high-content siRNA screening identifies the Alzheimer's genetic risk factor FERMT2 as a major modulator of APP metabolism.
Application : WB
Reactivity : Human
Submit a Reference
Package List Price ($)
$ 399