GeneTex

  • Country / Location Selection

United States (US)

Mig-6 antibody [N1C1]

Anti-Mig-6 antibody [N1C1] used in Western Blot (WB). GTX118238
Anti-Mig-6 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX118238

Cat No. GTX118238

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all Mig-6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Mig-6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721052

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ERBB receptor feedback inhibitor 1 , GENE-33 , MIG-6 , MIG6 , RALT

Background

ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al., 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33, which is induced during cell stress and mediates cell signaling (Makkinje et al., 2000 [PubMed 10749885]; Fiorentino et al., 2000 [PubMed 11003669]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Mig-6 antibody [N1C1] used in Western Blot (WB). GTX118238

GTX118238 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX118238 diluted at 1:1000

Anti-Mig-6 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX118238

GTX118238 IHC-P Image

Mig-6 antibody [N1C1] detects Mig-6 protein at cytoplasm in mouse placenta by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
Mig-6 antibody [N1C1] (GTX118238) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yu Y et al. Cancer Sci 2020; 111 (7) : 2297-2309 Long noncoding RNA ANRIL promotes the malignant progression of cholangiocarcinoma by epigenetically repressing ERRFI1 expression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Mig-6 antibody [N1C1] (GTX118238). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169