*Optimal dilutions/concentrations should be determined by the researcher.
|0.5-1ug/ml for 30 minutes at RT
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.
Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Delipidated human milk fat globule membrane preparation
Ab purified from Bioreactor Concentrate by Protein A/G
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Milk Fat Globule-Egf Factor 8 Protein,Ba46,Edil1,Hmfg,Hst19888,Mfg-E8,Mfgm,Oacgd3S,Sed1,Spag10,Hp47,Mfge8