APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:100-1:200 |
| 1:100 |
| 1/20 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
The antibody can also react with the steroid binding domain for aldosterone on other receptors.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.4 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Aldosterone-3
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear receptor subfamily 3 group C member 2 , MCR , MLR , MR , NR3C2VIT
Cellular Localization
Cytoplasm,Nucleus
Background
This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Database
Research Area
DATA IMAGES
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774](/templates/default/js/lightbox/images/loading.gif "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774")
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GTX22774 ICC/IF Image
ICC/IF analysis of HEK293 cells using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
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GTX22774 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100
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GTX22774 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100
|
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|
GTX22774 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
|
|
|
GTX22774 IHC-P Image
IHC-P analysis of human kidney tissue using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
|
|
|
GTX22774 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
|
REFERENCE
There are currently no references for Mineralocorticoid Receptor antibody [H10E4C9F] (GTX22774). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Mineralocorticoid Receptor antibody [H10E4C9F] (GTX22774). Be the first to share your experience with this product.
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774](/upload/website/prouct_img/small/GTX22774/GTX22774_426_ICC-IF_w_23060620_642.webp "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774")
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in IHC (Paraffin sections) (IHC-P). GTX22774](/upload/website/prouct_img/small/GTX22774/GTX22774_1101_IHC-P_w_23060620_472.webp "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in IHC (Paraffin sections) (IHC-P). GTX22774")
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in IHC (Paraffin sections) (IHC-P). GTX22774](/upload/website/prouct_img/small/GTX22774/GTX22774_1099_IHC-P_w_23060620_185.webp "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in IHC (Paraffin sections) (IHC-P). GTX22774")
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774](/upload/website/prouct_img/small/GTX22774/GTX22774_427_ICC-IF_w_23060620_861.webp "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774")
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in IHC (Paraffin sections) (IHC-P). GTX22774](/upload/website/prouct_img/small/GTX22774/GTX22774_1100_IHC-P_w_23060620_601.webp "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in IHC (Paraffin sections) (IHC-P). GTX22774")
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774](/upload/website/prouct_img/small/GTX22774/GTX22774_428_ICC-IF_w_23060620_819.webp "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774")
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774](/upload/website/prouct_img/normal/GTX22774/GTX22774_426_ICC-IF_w_23060620_642.webp "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774")
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in IHC (Paraffin sections) (IHC-P). GTX22774](/upload/website/prouct_img/normal/GTX22774/GTX22774_1101_IHC-P_w_23060620_472.webp "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in IHC (Paraffin sections) (IHC-P). GTX22774")
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in IHC (Paraffin sections) (IHC-P). GTX22774](/upload/website/prouct_img/normal/GTX22774/GTX22774_1099_IHC-P_w_23060620_185.webp "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in IHC (Paraffin sections) (IHC-P). GTX22774")
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774](/upload/website/prouct_img/normal/GTX22774/GTX22774_427_ICC-IF_w_23060620_861.webp "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774")
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in IHC (Paraffin sections) (IHC-P). GTX22774](/upload/website/prouct_img/normal/GTX22774/GTX22774_1100_IHC-P_w_23060620_601.webp "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in IHC (Paraffin sections) (IHC-P). GTX22774")
![Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774](/upload/website/prouct_img/normal/GTX22774/GTX22774_428_ICC-IF_w_23060620_819.webp "Anti-Mineralocorticoid Receptor antibody [H10E4C9F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22774")