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Mist1 antibody [6E8/A12/C11P1]

Anti-Mist1 antibody [6E8/A12/C11P1] used in IHC (Paraffin sections) (IHC-P). GTX79479
Anti-Mist1 antibody [6E8/A12/C11P1] used in Western Blot (WB). GTX79479

Cat. No. GTX79479

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6E8/A12/C11P1

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Mist1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:500
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse Mist1, C-terminus residues 175-197.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11178878

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

basic helix-loop-helix family member a15 , BHLHB8 , MIST1

Cellular Localization

Nucleus

Background

Mist 1 plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. This protein also negatively regulates bHLH-mediated transcription through an N-terminal repressor domain, and serves as a key regulator of acinar cell function, stability, and identity. Mist 1 is also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport, and may function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages.

Database

Research Area

DATA IMAGES

Anti-Mist1 antibody [6E8/A12/C11P1] used in IHC (Paraffin sections) (IHC-P). GTX79479

GTX79479 IHC-P Image

IHC-P analysis of mouse pancreas tissue using GTX79479 Mist1 antibody [6E8/A12/C11P1].

Anti-Mist1 antibody [6E8/A12/C11P1] used in Western Blot (WB). GTX79479

GTX79479 WB Image

WB analysis of whole cell extracts (30 μg lysate) of Mouse Pancreas (Lane 1), Panc1 (Lane 2) and Mouse spleen (Lane 3) using GTX79479 Mist1 antibody [6E8/A12/C11P1].
Dilution : 1:100-1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399