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Mitfa antibody

Anti-Mitfa antibody used in IHC (Whole mount) (IHC-Wm). GTX128263
Anti-Mitfa antibody used in Western Blot (WB). GTX128263

Cat. No. GTX128263

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Zebrafish, Carp
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Mitfa products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

Positive Control

zebrafish muscle

Predict Reactivity

Human, Mouse, Rat, Bovine, Dog, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Mitfa. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885742

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

melanocyte inducing transcription factor a , nacre , z3A.1

Database

Research Area

DATA IMAGES

Anti-Mitfa antibody used in IHC (Whole mount) (IHC-Wm). GTX128263

GTX128263 IHC-Wm Image

mitfa antibody detects mitfa protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
mitfa antibody (GTX128263) dilution: 1:200.
Anti-Mitfa antibody used in Western Blot (WB). GTX128263

GTX128263 WB Image

Mitfa antibody detects Mitfa protein by western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Mitfa antibody (GTX128263) at a dilution of 1:1000.

REFERENCE

Application Reference

Park JH et al. Lasers Surg Med 2021; (Epub) Identification of the fate and regenerative mechanism of zebrafish melanocyte progenitor cells and melanocytes after laser-induced pigment ablation.
Application : WB
Reactivity : Zebrafish
Zhang Y et al. BMC Genet 2017; 18 (1) : 95 Comparative Transcriptome and DNA methylation analyses of the molecular mechanisms underlying skin color variations in Crucian carp (Carassius carassius L.).
Application : WB
Reactivity : Carp
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169