‧ Detection method- Fluorecence microscopy and Fluorescent plate reader
‧ Sample type- Live cells
‧ Species reactivity- Mammalian
‧ Applications- Disruption of mitochondrial transmembrane potential is one of the earliest intracellular events that occur upon induction of apoptosis. The MitoLume Mitochondrial Apoptosis Detection Kit provides a simple and sensitive in vitro assay for detecting the mitochondrial changes in apoptosis. It is highly sensive and detects apoptosis in living cells.
Features and Benefits
‧ Simple one-step procedure; takes only 30 minutes
‧ Fast and convenient
‧ The assay utilizes a novel cationic dye (called MitoLume) that fluoresces differently in healthy versus apoptotic cells. In healthy cells MitoLume accumulates and aggregates in the mitochondria-fluorescing bright red. In apoptotic cells a shift in mitochondrial transmembrane potential prevents the dye from accumulating in the mitochondria. Instead it remains in the cytoplasm in its monomer form-fluorescing green.
GTX85581 FACS Image
MitoLume Effectively Detects Disruption of Mitochondrial Transmembrane Potential in Apoptotic Cells. Apoptosis was induced in Jurkat cells by 2 microM camptothecin for various hours as indicated. Cells were incubated with MitoLume for 15 minutes and analyzed by flow cytometry using the FITC channel.
GTX85581 ICC/IF Image
Detection of Change in Mitochondrial Transmembrane Potential by MitoLume and Fluorescence Microscopy. The photograph shows examples of cells scored for intact and apoptotic cells using MitoLume probe. The apoptotic cell (left panel) shows diffused green fluorescence, while the healthy cell (right panel) shows punctate red fluorescence.
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