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MitoLume Mitochondrial Apoptosis Detection Kit

MitoLume Mitochondrial Apoptosis Detection Kit. GTX85581
MitoLume Mitochondrial Apoptosis Detection Kit. GTX85581

Cat No. GTX85581

Application ICC/IF, FACS
Package
100 assay ($429)
PRODUCT

Product Description

Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoLume? Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential. The kit utilizes MitoLume?, a cationic dye that fluoresces differently in healthy vs apoptotic cells. In healthy cells, MitoLume accumulates and aggregates in the mitocondria, giving off a bright red fluorescence. In apoptotic cells, MitoLume cannot aggregate in the mitochondria due to the altered mitochondrial transmembrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530+ 30 nm) and (optional) PI channel for red aggregates (Em = 488/590+ 42 nm).

‧ Detection method- Fluorecence microscopy and Fluorescent plate reader
‧ Sample type- Live cells
‧ Species reactivity- Mammalian
‧ Applications- Disruption of mitochondrial transmembrane potential is one of the earliest intracellular events that occur upon induction of apoptosis. The MitoLume Mitochondrial Apoptosis Detection Kit provides a simple and sensitive in vitro assay for detecting the mitochondrial changes in apoptosis. It is highly sensive and detects apoptosis in living cells.

Features and Benefits
‧ Simple one-step procedure; takes only 30 minutes
‧ Fast and convenient
‧ The assay utilizes a novel cationic dye (called MitoLume) that fluoresces differently in healthy versus apoptotic cells. In healthy cells MitoLume accumulates and aggregates in the mitochondria-fluorescing bright red. In apoptotic cells a shift in mitochondrial transmembrane potential prevents the dye from accumulating in the mitochondria. Instead it remains in the cytoplasm in its monomer form-fluorescing green.

Kit Contents:
MitoLume Reagent
Incubation Buffer

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.
PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

mitochondrial membrane potential assay, mitochondrial potential assay, Mitochondrial apoptosis assay
DATA IMAGES
MitoLume Mitochondrial Apoptosis Detection Kit. GTX85581

GTX85581 FACS Image

MitoLume Effectively Detects Disruption of Mitochondrial Transmembrane Potential in Apoptotic Cells. Apoptosis was induced in Jurkat cells by 2 microM camptothecin for various hours as indicated. Cells were incubated with MitoLume for 15 minutes and analyzed by flow cytometry using the FITC channel.

MitoLume Mitochondrial Apoptosis Detection Kit. GTX85581

GTX85581 ICC/IF Image

Detection of Change in Mitochondrial Transmembrane Potential by MitoLume and Fluorescence Microscopy. The photograph shows examples of cells scored for intact and apoptotic cells using MitoLume probe. The apoptotic cell (left panel) shows diffused green fluorescence, while the healthy cell (right panel) shows punctate red fluorescence.

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Package List Price ($)
$ 429