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Mitochondrial Ferritin antibody [N1C3]

Anti-Mitochondrial Ferritin antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117281
Anti-Mitochondrial Ferritin antibody [N1C3] used in Western Blot (WB). GTX117281
Anti-Mitochondrial Ferritin antibody [N1C3] used in Western Blot (WB). GTX117281

Cat. No. GTX117281

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Mitochondrial Ferritin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722706

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ferritin mitochondrial , MTF

Background

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.

Database

Research Area

DATA IMAGES

Anti-Mitochondrial Ferritin antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117281

GTX117281 IHC-P Image

FTMT antibody [N1C3] detects FTMT protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
FTMT antibody [N1C3] (GTX117281) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Mitochondrial Ferritin antibody [N1C3] used in Western Blot (WB). GTX117281

GTX117281 WB Image

Sample (30 μg of whole cell lysate)
A: U87-MG
12% SDS PAGE
GTX117281 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Mitochondrial Ferritin antibody [N1C3] used in Western Blot (WB). GTX117281

GTX117281 WB Image

Human tissue extract was separated by 12% SDS-PAGE, and the membrane was blotted with Mitochondrial Ferritin antibody [N1C3] (GTX117281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169