GeneTex
United States (US)

Mitofilin antibody

GTX115523_40632_IFA_18121410_674.jpg
GTX115523_40632_WB_18121410_752.jpg

Cat No. GTX115523

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

U87-MG , MCF-7

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Mitofilin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Inner Membrane Mitochondrial Protein,Hmp,Minos2,Mic60,P87,P87/89,P89,Pig4,Pig52,Immt

Cellular Localization

Mitochondrion inner membrane

Database

Research Area

DATA IMAGES
GTX115523_40632_IFA_18121410_674.jpg

GTX115523 ICC/IF Image

Mitofilin antibody detects Mitofilin protein at mitochondria by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: Mitofilin protein stained by Mitofilin antibody (GTX115523) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX115523_40632_WB_18121410_752.jpg

GTX115523 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX115523 diluted at 1:1000

REVIEW

Mitofilin antibody Cat No. GTX115523

Rating ( Average 5 based on 1 users reviews)
Application
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 28-Jul-2016
Score :
Application Tested : IP
Sample Species : Hu
Sample : MM.1S cells
Amount used : 300μg for IP
Blocking : 5% milk in TBS, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Figure Note : Immunoprecipitation of Mitofilin protein from MM.1S whole cell extracts using 3 μg of Mitofilin antibody (GTX115523) and detected by Mitofilin antibody of WB.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169