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Mitofilin antibody

Anti-Mitofilin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115523
Anti-Mitofilin antibody used in Western Blot (WB). GTX115523

Cat. No. GTX115523

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Mitofilin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Mitofilin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166340

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

inner membrane mitochondrial protein , HMP , MICOS60 , MINOS2 , Mic60 , P87 , P87/89 , P89 , PIG4 , PIG52

Cellular Localization

Mitochondrion inner membrane

Database

Research Area

DATA IMAGES

Anti-Mitofilin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115523

GTX115523 ICC/IF Image

Mitofilin antibody detects Mitofilin protein at mitochondria by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: Mitofilin protein stained by Mitofilin antibody (GTX115523) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Mitofilin antibody used in Western Blot (WB). GTX115523

GTX115523 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX115523 diluted at 1:1000

REFERENCE

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REVIEW

Mitofilin antibody Cat. No. GTX115523

Rating
( Average 5 based on 1 users reviews)
Application
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Mitofilin antibody used in Immunoprecipitation (IP). GTX115523
Date : Anonymous submitted on 28-Jul-2016
Score :
Application Tested : IP
Sample Species : Hu
Sample : MM.1S cells
Amount used : 300μg for IP
Blocking : 5% milk in TBS, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Immunoprecipitation of Mitofilin protein from MM.1S whole cell extracts using 3 μg of Mitofilin antibody (GTX115523) and detected by Mitofilin antibody of WB.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169