Mitofilin antibody

Cat No. GTX115523

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IP
Reactivity	Human

See all Mitofilin products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IP	Assay dependent

Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

U87-MG , MCF-7

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Mitofilin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Inner Membrane Mitochondrial Protein,Hmp,Minos2,Mic60,P87,P87/89,P89,Pig4,Pig52,Immt

Cellular Localization

Mitochondrion inner membrane

Database

Gene ID: 10989
UniProt: Q16891

Research Area

DATA IMAGES

GTX115523 ICC/IF Image

Mitofilin antibody detects Mitofilin protein at mitochondria by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: Mitofilin protein stained by Mitofilin antibody (GTX115523) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX115523 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX115523 diluted at 1:1000

REVIEW