Western blot using GeneTex's affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000.