GeneTex

  • Country / Location Selection

United States (US)

Mlf1 Interacting Protein antibody

KO/KD Overexpression
Anti-Mlf1 Interacting Protein antibody used in Western Blot (WB). GTX48731

Cat. No. GTX48731

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Human, Bovine, Dog, Chimpanzee
Package
50 μg ($399)
See all Mlf1 Interacting Protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ELISA 1:5000 - 1:25000
IHC Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centromere protein U , CENP50 , CENPU50 , KLIP1 , MLF1IP , PBIP1

Cellular Localization

Cytoplasm,Nucleus

Background

Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition.Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

Database

Research Area

DATA IMAGES

Anti-Mlf1 Interacting Protein antibody used in Western Blot (WB). GTX48731

GTX48731 WB Image

KO/KD Overexpression

Western blot using GeneTex's affinity purified anti-MLF1IP antibody shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (Lane 1). Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for PBIP1/MLF1IP.

REFERENCE

There are currently no references for Mlf1 Interacting Protein antibody (GTX48731). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Mlf1 Interacting Protein antibody (GTX48731). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399