GeneTex
United States (US)

Moesin antibody [C2C3], C-term

GTX101708_40198_WB_M_19011506_416.jpg
GTX101708_40198_IFA_19011506_369.jpg
GTX101708_WB_19011506_590.jpg
GTX101708_40198_20150413_IP_19011506_545.jpg
GTX101708_40198_20160301_IHC-P_R_19011506_649.jpg

Cat No. GTX101708

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

NT2D1 , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Moesin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MSN antibody, membrane-organizing extension spike protein antibody, moesin antibody

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) , Cytoplasm , cytoskeleton (By similarity) , Apical cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) , Cell projection , microvillus membrane; Periphe

Background

Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101708_40198_WB_M_19011506_416.jpg

GTX101708 WB Image

Moesin antibody [C2C3], C-term detects MSN protein by Western blot analysis.
A. 30 µg GL261 whole cell lysate/extract
B. 30 µg C8D30 whole cell lysate/extract
C. 30 µg NIH-3T3 whole cell lysate/extract
D. 30 µg BCL-1 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
F. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Moesin antibody [C2C3], C-term (GTX101708) dilution: 1:1000

GTX101708_40198_IFA_19011506_369.jpg

GTX101708 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Moesin(GTX101708) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:500.

GTX101708_WB_19011506_590.jpg

GTX101708 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX101708 diluted at 1:1000

GTX101708_40198_20150413_IP_19011506_545.jpg

GTX101708 IP Image

Immunoprecipitation of Moesin protein from A431 whole cell extracts using 5 μg of Moesin antibody [C2C3], C-term (GTX101708).
Western blot analysis was performed using Moesin antibody [C2C3], C-term (GTX101708).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101708_40198_20160301_IHC-P_R_19011506_649.jpg

GTX101708 IHC-P Image

Moesin antibody [C2C3], C-term detects Moesin protein at cell membrane in rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Moesin antibody [C2C3], C-term (GTX101708) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169