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Moesin antibody [C2C3], C-term

Anti-Moesin antibody [C2C3], C-term used in Western Blot (WB). GTX101708
Anti-Moesin antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101708
Anti-Moesin antibody [C2C3], C-term used in Western Blot (WB). GTX101708
Anti-Moesin antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101708
Anti-Moesin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101708

Cat No. GTX101708

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

NT2D1 , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Moesin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

moesin , HEL70 , IMD50

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytoskeleton , Apical cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell projection , microvillus membrane; Periphe

Background

Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Moesin antibody [C2C3], C-term used in Western Blot (WB). GTX101708

GTX101708 WB Image

Moesin antibody [C2C3], C-term detects MSN protein by Western blot analysis.
A. 30 µg GL261 whole cell lysate/extract
B. 30 µg C8D30 whole cell lysate/extract
C. 30 µg NIH-3T3 whole cell lysate/extract
D. 30 µg BCL-1 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
F. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Moesin antibody [C2C3], C-term (GTX101708) dilution: 1:1000

Anti-Moesin antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101708

GTX101708 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Moesin(GTX101708) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:500.

Anti-Moesin antibody [C2C3], C-term used in Western Blot (WB). GTX101708

GTX101708 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX101708 diluted at 1:1000

Anti-Moesin antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101708

GTX101708 IP Image

Immunoprecipitation of Moesin protein from A431 whole cell extracts using 5 μg of Moesin antibody [C2C3], C-term (GTX101708).
Western blot analysis was performed using Moesin antibody [C2C3], C-term (GTX101708).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Moesin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101708

GTX101708 IHC-P Image

Moesin antibody [C2C3], C-term detects Moesin protein at cell membrane in rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Moesin antibody [C2C3], C-term (GTX101708) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169