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Moesin antibody [C2C3], C-term

KO/KD
Anti-Moesin antibody [C2C3], C-term used in Western Blot (WB). GTX101708
Anti-Moesin antibody [C2C3], C-term used in Western Blot (WB). GTX101708
Anti-Moesin antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101708
Anti-Moesin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101708
Anti-Moesin antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101708
Anti-Moesin antibody [C2C3], C-term used in Western Blot (WB). GTX101708

Cat. No. GTX101708

Reference 5
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Moesin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Moesin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618789

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

moesin , HEL70 , IMD50

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytoskeleton , Apical cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell projection , microvillus membrane; Periphe

Background

Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Moesin antibody [C2C3], C-term used in Western Blot (WB). GTX101708

GTX101708 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Moesin antibody [C2C3], C-term (GTX101708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Moesin antibody [C2C3], C-term used in Western Blot (WB). GTX101708

GTX101708 WB Image

Moesin antibody [C2C3], C-term detects MSN protein by Western blot analysis.
A. 30 μg GL261 whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Moesin antibody [C2C3], C-term (GTX101708) dilution: 1:1000

Anti-Moesin antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101708

GTX101708 IP Image

Immunoprecipitation of Moesin protein from A431 whole cell extracts using 5 μg of Moesin antibody [C2C3], C-term (GTX101708).
Western blot analysis was performed using Moesin antibody [C2C3], C-term (GTX101708).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Moesin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101708

GTX101708 IHC-P Image

Moesin antibody [C2C3], C-term detects Moesin protein at cell membrane in rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Moesin antibody [C2C3], C-term (GTX101708) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Moesin antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101708

GTX101708 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Moesin(GTX101708) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:500.

Anti-Moesin antibody [C2C3], C-term used in Western Blot (WB). GTX101708

GTX101708 WB Image

The data was published in the journal Breast Cancer Res in 2014. PMID: 24512624

REFERENCE

Application Reference

Alshafie W et al. F1000Res 2023; 12 : 172 Identification of high-performing antibodies for Moesin for use in Western Blot, immunoprecipitation, and immunofluorescence.
Chang YT et al. Am J Cancer Res 2020; 10 (1) : 12-37 Pancreatic cancer-derived small extracellular vesical Ezrin regulates macrophage polarization and promotes metastasis.
Application : WB
Reactivity : Human
Ogiwara K et al. Biol Reprod 2016; A Dual Role for Melatonin in Medaka Ovulation: Ensuring Prostaglandin Synthesis and Actin Cytoskeleton Rearrangement in Follicular Cells.
Application : WB
Babina IS et al. Breast Cancer Res 2014; 16 (1) : R19 A novel mechanism of regulating breast cancer cell migration via palmitoylation-dependent alterations in the lipid raft affiliation of CD44.
Application : WB
Reactivity : Human
Chen YH et al. J Proteomics 2012; 77 : 111-28 Effect of high glucose on secreted proteome in cultured retinal pigmented epithelium cells: its possible relevance to clinical diabetic retinopathy.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

Moesin antibody [C2C3], C-term Cat. No. GTX101708

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Moesin antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101708
Date : Anonymous submitted on 06-Jan-2021
Score :
Application Tested : IP
Sample Species : Hu
Sample : HeLa cells
Figure Note : Immunoprecipitation of Moesin protein from HeLa cells using 1 μg of GTX101708 Moesin antibody [C2C3], C-term.
SM=10% starting material
UB=10% unbound fraction
Anti-Moesin antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101708
Date : Anonymous submitted on 06-Jan-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa cells
Blocking : 5% BSA, 5% goat serum and 0.01% Triton X-100 in PBS, 25°C, 0.5Hr
Fixation : 4% PFA in PBS, 0.25Hr
Permeabilization /Antigen retrieval : 0.1% Triton X-100 in PBS, 25°C, 0.2Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Parental and KO cells are outlined with yellow and red dashed line, respectively.
Bars = 40 μm.
Anti-Moesin antibody [C2C3], C-term used in Western Blot (WB). GTX101708
Date : Anonymous submitted on 06-Jan-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 25 μg
Blocking : 5% milk, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169