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Monoamine Oxidase B antibody [N1N3]

Orthogonal
Anti-Monoamine Oxidase B antibody [N1N3] used in Western Blot (WB). GTX113771
Anti-Monoamine Oxidase B antibody [N1N3] used in Western Blot (WB). GTX113771
Anti-Monoamine Oxidase B antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113771
Anti-Monoamine Oxidase B antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113771

Cat. No. GTX113771

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Monoamine Oxidase B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

HepG2 , rat liver

Predict Reactivity

Bovine, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Monoamine Oxidase B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037455

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

monoamine oxidase B

Cellular Localization

Mitochondrion outer membrane

Background

The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Monoamine Oxidase B antibody [N1N3] used in Western Blot (WB). GTX113771

GTX113771 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N1N3] (GTX113771) diluted at 1:3000.

Anti-Monoamine Oxidase B antibody [N1N3] used in Western Blot (WB). GTX113771

GTX113771 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX113771 diluted at 1:3000

Anti-Monoamine Oxidase B antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113771

GTX113771 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using Monoamine Oxidase B(GTX113771) antibody at 1:200 dilution.

Anti-Monoamine Oxidase B antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113771

GTX113771 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Monoamine Oxidase B(GTX113771) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Wang H et al. NPJ Parkinsons Dis 2023; 9 (1) : 1 C/EBPβ/AEP is age-dependently activated in Parkinson's disease and mediates α-synuclein in the gut and brain.
Application : WB
Reactivity : Mouse
Neumann J et al. Front Pharmacol 2021; 12 : 582916 Histamine can be Formed and Degraded in the Human and Mouse Heart.
Application : WB, IHC-P
Reactivity : Mouse
Kang SS et al. J Clin Invest 2020; 130 (1) : 422-437 Norepinephrine metabolite DOPEGAL activates AEP and pathological Tau aggregation in locus coeruleus.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169