Monoamine Oxidase B antibody [N2C3]

Name: Monoamine Oxidase B antibody [N2C3]
Brand: GeneTex
SKU: GTX105970
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Monoamine Oxidase B antibody [N2C3] used in Western Blot (WB). GTX105970

Anti-Monoamine Oxidase B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105970

Anti-Monoamine Oxidase B antibody [N2C3] used in ELISA (ELISA). GTX105970

Anti-Monoamine Oxidase B antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105970

Cat. No. GTX105970

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA, IHC, Sandwich ELISA
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Monoamine Oxidase B products

View product citations for antibody GTX105970 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
ELISA	1:1000-1:10000
IHC	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture: GTX105970 , Detection: GTX89368

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Mouse liver

Predict Reactivity

Rat, Bovine, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Monoamine Oxidase B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950856

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

monoamine oxidase B

Cellular Localization

Mitochondrion outer membrane

Background

The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq]

Database

Gene ID: 4129 MAOB
UniProt: P27338 MAOB

Research Area

DATA IMAGES

GTX105970 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105970 IHC-P Image

Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105970 IHC-P Image

Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105970 ELISA Image

ELISA detection of MAOB using GTX105970 for capture at a concentration of 2.5 μg/mL and GTX89368 for detection at a concentration of 0.5 μg/mL.

GTX105970 ICC/IF Image

Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX105970 WB Image

HepG2 whole cell and mitochondria extracts (8.1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105970 IHC-P Image

The data was published in the journal Int J Mol Sci in 2020.PMID: 32316576

REFERENCE

Application Reference

Ping-Ho Chen et al. Cancers (Basel) 2021; 13 (13) Genetic and Proteinic Linkage of MAO and COMT with Oral Potentially Malignant Disorders and Cancers of the Oral Cavity and Pharynx.

Application : WB

Reactivity : Human

Yang YC et al. Int J Mol Sci 2020; 21 (8) Monoamine Oxidase B Expression Correlates with a Poor Prognosis in Colorectal Cancer Patients and Is Significantly Associated with Epithelial-to-Mesenchymal Transition-Related Gene Signatures.

Liu Y et al. Mol Neurobiol 2019; (Epub) Attenuation of Ischemic Stroke-Caused Brain Injury by a Monoamine Oxidase Inhibitor Involves Improved Proteostasis and Reduced Neuroinflammation.

Application : WB

Reactivity : Mouse

Seong Su Kang et al. bioRxiv 2019; (Epub) The Norepinephrine Metabolite DOPEGAL Confers Locus Coeruleus Tau Vulnerability and Propagation via Asparagine Endopeptidase.

Application : WB

Reactivity : Human

Kang SS et al. EMBO J 2018; (Epub) α-Synuclein stimulation of monoamine oxidase-B and legumain protease mediates the pathology of Parkinson's disease.

Application : WB

Reactivity : Mouse

Submit a Reference

REVIEW