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United States (US)

Monoamine Oxidase B antibody [N2C3]

GTX105970_43300_20181205_ICC_IF_19021719_978.jpg
GTX105970_39932_WB_M_Liver_19011506_787.jpg
MAOB_ELISA-PAIR_19011506_268.jpg
GTX105970_39932_IHC_19011506_404.jpg
GTX105970_43300_20180810_WB_19011506_953.jpg
GTX105970_39932_20150402_IHC_M_19011506_304.jpg

Cat No. GTX105970

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
IHC Assay dependent
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

HepG2 , mouse liver

Predict Reactivity

Rat, Bovine, Dog, Guinea pig, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Monoamine Oxidase B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Monoamine Oxidase B,Maob

Cellular Localization

Mitochondrion outer membrane

Background

The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105970_43300_20181205_ICC_IF_19021719_978.jpg

GTX105970 ICC/IF Image

Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX105970_39932_WB_M_Liver_19011506_787.jpg

GTX105970 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse liver
7.5% SDS PAGE
GTX105970 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MAOB_ELISA-PAIR_19011506_268.jpg

GTX105970 ELISA Image

ELISA detection of MAOB using GTX105970 for capture at a concentration of 2.5 µg/mL and GTX89368 for detection at a concentration of 0.5 µg/mL.

GTX105970_39932_IHC_19011506_404.jpg

GTX105970 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Monoamine Oxidase B(GTX105970) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105970_43300_20180810_WB_19011506_953.jpg

GTX105970 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105970_39932_20150402_IHC_M_19011506_304.jpg

GTX105970 IHC-P Image

Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

Monoamine Oxidase B antibody [N2C3] Cat No. GTX105970

Rating ( Average 5 based on 1 users reviews)
Application
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 29-May-2018
Score :
Application Tested : IHC
Sample : Drosophila brain
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, overnightHr
Figure Note : Human gene express in drosophila tissue.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169