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Monoglyceride lipase antibody

Anti-Monoglyceride lipase antibody used in Western Blot (WB). GTX103517
Anti-Monoglyceride lipase antibody used in IHC (Paraffin sections) (IHC-P). GTX103517
Anti-Monoglyceride lipase antibody used in IHC (Paraffin sections) (IHC-P). GTX103517
Anti-Monoglyceride lipase antibody used in IHC (Paraffin sections) (IHC-P). GTX103517

Cat. No. GTX103517

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Monoglyceride lipase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Monoglyceride lipase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615951

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

monoglyceride lipase , HU-K5 , HUK5 , MAGL , MGL

Background

Monoglyceride lipase (MGLL; EC 3.1.1.23) functions together with hormone-sensitive lipase (LIPE; MIM 151750) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL; MIM 238600) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides (Karlsson et al., 2001 [PubMed 11470505]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Monoglyceride lipase antibody used in Western Blot (WB). GTX103517

GTX103517 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoglyceride lipase antibody (GTX103517) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Monoglyceride lipase antibody used in IHC (Paraffin sections) (IHC-P). GTX103517

GTX103517 IHC-P Image

Monoglyceride lipase antibody detects MGLL protein at nucleus on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
Monoglyceride lipase antibody (GTX103517) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Monoglyceride lipase antibody used in IHC (Paraffin sections) (IHC-P). GTX103517

GTX103517 IHC-P Image

Monoglyceride lipase antibody detects Monoglyceride lipase protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Monoglyceride lipase antibody (GTX103517) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Monoglyceride lipase antibody used in IHC (Paraffin sections) (IHC-P). GTX103517

GTX103517 IHC-P Image

Monoglyceride lipase antibody detects Monoglyceride lipase protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Monoglyceride lipase antibody (GTX103517) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Nakatsu Y et al. Metabolism 2021; 115 : 154459 Prolyl isomerase Pin1 interacts with adipose triglyceride lipase and negatively controls both its expression and lipolysis.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169