GeneTex
United States (US)

Monoglyceride lipase antibody

GTX103517_40198_IHC_19011506_533.jpg
GTX103517_40198_WB_M_White adipose tissue_19011720_247.jpg
GTX103517_40198_IHC_R_19011506_715.jpg
GTX103517_WB_19011506_291.jpg
GTX103517_40198_IHC_M_19011506_564.jpg

Cat No. GTX103517

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
See all Monoglyceride lipase products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLa , HepG2 , mouse adipose

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Monoglyceride lipase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Monoglyceride Lipase,Hu-K5,Huk5,Magl,Mgl,Mgll

Background

Monoglyceride lipase (MGLL; EC 3.1.1.23) functions together with hormone-sensitive lipase (LIPE; MIM 151750) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL; MIM 238600) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides (Karlsson et al., 2001 [PubMed 11470505]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX103517_40198_IHC_19011506_533.jpg

GTX103517 IHC-P Image

Monoglyceride lipase antibody detects MGLL protein at nucleus on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
Monoglyceride lipase antibody (GTX103517) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX103517_40198_WB_M_White adipose tissue_19011720_247.jpg

GTX103517 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse white adipose tissue
10% SDS PAGE
GTX103517 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX103517_40198_IHC_R_19011506_715.jpg

GTX103517 IHC-P Image

Monoglyceride lipase antibody detects Monoglyceride lipase protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Monoglyceride lipase antibody (GTX103517) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX103517_WB_19011506_291.jpg

GTX103517 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
B: HepG2 (GTX27900)
10% SDS PAGE
GTX103517 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX103517_40198_IHC_M_19011506_564.jpg

GTX103517 IHC-P Image

Monoglyceride lipase antibody detects Monoglyceride lipase protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Monoglyceride lipase antibody (GTX103517) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169