APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , HepG2 , mouse adipose
Predict Reactivity
Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.39 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Monoglyceride lipase. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10615951
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
monoglyceride lipase , HU-K5 , HUK5 , MAGL , MGL
Background
Monoglyceride lipase (MGLL; EC 3.1.1.23) functions together with hormone-sensitive lipase (LIPE; MIM 151750) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL; MIM 238600) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides (Karlsson et al., 2001 [PubMed 11470505]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX103517 IHC-P Image
Monoglyceride lipase antibody detects MGLL protein at nucleus on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
Monoglyceride lipase antibody (GTX103517) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103517 IHC-P Image
Monoglyceride lipase antibody detects Monoglyceride lipase protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Monoglyceride lipase antibody (GTX103517) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103517 IHC-P Image
Monoglyceride lipase antibody detects Monoglyceride lipase protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Monoglyceride lipase antibody (GTX103517) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103517 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoglyceride lipase antibody (GTX103517) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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![Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143](/upload/website/prouct_img/small/GTX628143/GTX628143_41113_20191129_WB_Fraction_19120122_515.jpg "Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143")
