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Mouse AHA-1 protein, His tag (active)

Mouse AHA-1 protein, His tag (active). GTX00428-pro

Cat. No. GTX00428-pro

Application

 Functional Assay

Species

 Mouse
Package
50 μg ($509)
See all AHA-1 protein products

APPLICATION

Application Note

2 μM AHA1 Protein generated a 9-fold ATPase activation of 2 μM HSP90 (His-tagged HSP90 beta) in 33 mM HEPES (pH 7.2) with 30 mM NaCl, 5 mM MgCl₂, 1 mM DTT, 1.5 mM ATP in a 100 μl reaction at 37 ºC. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)

Observed MW

38 kDa.

PROPERTIES

Form

Liquid

Buffer

25mM HEPES buffer (pH7.2), 100mM KoAc, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full-length protein, N-terminal His-Tag; NP_666148.1

Expression System

E. coli

Purification

Affinity Purified

Purity

> 90%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AHA1, activator of heat shock protein ATPase 1 , BC023857 , p38

Database

Research Area

DATA IMAGES

Mouse AHA-1 protein, His tag (active). GTX00428-pro

GTX00428-pro Image

SDS-PAGE analysis of GTX00428-pro Mouse AHA-1 protein (active).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 509