GeneTex
  • Country / Location Selection

United States (US)

Mouse Alkaline Phosphatase (Tissue Non-Specific) protein, His tag (active)

Mouse Alkaline Phosphatase (Tissue Non-Specific)  protein, His tag (active). GTX66924-pro

Cat No. GTX66924-pro

Application

Functional Assay

Species

Mouse
Package
10 μg ($219)

APPLICATION

Application Note

Specific activity is > 46000 pmol/min/μg and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25ºC.

Observed MW

50-70 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4) with 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Region/Sequence

C-terminal His-Tag; Length: 493 a.a. Sequence: VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVT AARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTR CNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQL MHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GLDLISIWKS FKPRHKHSHY VWNRTELLAL DPSRVDYLLG LFEPGDMQYE LNRNNLTDPS LSEMVEVALR ILTKNLKGFF LLVEGGRIDH GHHEGKAKQA LHEAVEMDQA IGKAGAMTSQ KDTLTVVTAD HSHVFTFGGY TPRGNSIFGL APMVSDTDKK PFTAILYGNG PGYKVVDGER ENVSMVDYAH NNYQAQSAVP LRHETHGGED VAVFAKGPMA HLLHGVHEQN YIPHVMAYAS CIGANLDHCA WAGSGLEHHH HHH

Expression System

Baculovirus

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

alkaline phosphatase, liver/bone/kidney , ALP , APTNAP , Akp-2 , Akp2 , TNAP , TNSALP

Background

This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. The mature peptide maintains the ratio of inorganic phosphate to inorganic pyrophosphate required for bone mineralization. Mice that lack this enzyme show symptoms of osteomalacia, softening of the bones. In humans, mutations in this gene are associated with hypophosphatasia, an inherited metabolic bone disease in which deficiency of this enzyme inhibits bone mineralization leading to skeletal defects. Mutations in the mouse gene mirror the symptoms of human hypophosphatasia. A pseudogene of this gene is present on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Mouse Alkaline Phosphatase (Tissue Non-Specific)  protein, His tag (active). GTX66924-pro

GTX66924-pro Image

3 μg of GTX66924-pro Mouse Alkaline Phosphatase (Tissue Non-Specific) protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

There are currently no references for Mouse Alkaline Phosphatase (Tissue Non-Specific) protein, His tag (active) (GTX66924-pro). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Mouse Alkaline Phosphatase (Tissue Non-Specific) protein, His tag (active) (GTX66924-pro). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 219