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Mouse Annexin II protein (active)

Mouse Annexin II protein (active). GTX00332-pro
Mouse Annexin II protein (active). GTX00332-pro
Mouse Annexin II protein (active). GTX00332-pro

Cat No. GTX00332-pro

Application Functional Assay
Species Mouse
Package
10 μg ($259)
APPLICATION

Application Note

Annexin A2 (ANXA2) also known as annexin II is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. It is a calcium-dependent phospholipid-binding protein whose function is to help organize exocytosis of intracellular proteins to the extracellular domain. Besides, S100 Calcium Binding Protein A10 (S100A10) has been identified as an interactor of ANXA2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ANXA2 and recombinant mouse S100A10. Briefly, ANXA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to S100A10-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ANXA2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of ANXA2 and S100A10 was in a dose dependent manner.

Observed MW

42 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Asp339 (NP_031611.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

annexin A2,AW215814,Cal1h

Database

Research Area

DATA IMAGES
Mouse Annexin II protein (active). GTX00332-pro

GTX00332-pro Functional Assay Image

Functional ELISA analysis of GTX00332-pro Mouse Annexin II protein (active) which can bind immobilized S100A10 protein.

Mouse Annexin II protein (active). GTX00332-pro

GTX00332-pro Image

SDS-PAGE analysis of GTX00332-pro Mouse Annexin II protein (active).

Mouse Annexin II protein (active). GTX00332-pro

GTX00332-pro Image

WB analysis of GTX00332-pro Mouse Annexin II protein (active).

REFERENCE
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