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Mouse Anti-Human IgG4 (Fc) antibody [HP6025] (HRP)

Cat. No. GTX75819

Reference 8

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HP6025

Isotype

 IgG1

Application

 IHC-P, IHC-Fr, ELISA, EIA

Reactivity

 Human
Package
100 μg ($499)
See all Human IgG4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P Assay dependent
IHC-Fr 1/40-1/80
ELISA 1/25,600-1/51,200
EIA Assay dependent
Not tested in other applications.

Product Note

This antibody recognizes the heavy chain of human IgG4, at an epitope in the Fc region. No cross-reactivity is observed with IgG1, IgG2, IgG3, IgM, IgA (Jefferis et al. 1985).

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% ProClin 300, 0.002%Methylisothiazolone, 0.002% Bromonitrodioxane

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified IgG4

Target Isotype

IgG4 Fc

Purification

Protein A purified

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_377048

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

immunoglobulin heavy constant gamma 4 (G4m marker) Antibody , IGHG4 Antibody

Research Area

REFERENCE

Application Reference

Yordanova IA et al. Life (Basel) 2021; 11 (2) The Worm-Specific Immune Response in Multiple Sclerosis Patients Receiving Controlled Trichuris suis Ova Immunotherapy.
Application : ELISA
Yonenaga Y et al. Oncol Lett 2016; 12 (3) : 1801-1805 Localized 18F-fluorodeoxyglucose uptake at the pancreatic head during remission phase of autoimmune pancreatitis: A case report.
Application : IHC-P
Reactivity : Human
Chao PZ et al. Oncotarget 2015; 6 (1) : 159-70 Dendritic cells respond to nasopharygeal carcinoma cells through annexin A2-recognizing DC-SIGN.
Otsuka K et al. Sci Rep 2015; 5 : 13928 Collagen Q and anti-MuSK autoantibody competitively suppress agrin/LRP4/MuSK signaling.
Reactivity : Human
O'Regan NL et al. Parasite Immunol 2014; Filariasis asymptomatically infected donors have lower levels of disialylated IgG compared to endemic normals.
Application : ELISA
Reactivity : Human
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SDS
PBS.pdf
Proclin.pdf
Package List Price ($)
$ 499