Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
1/40-1/80 |
1/25,600-1/51,200 |
Not tested in other applications.
Product Note
This antibody recognizes the heavy chain of human IgG4, at an epitope in the Fc region. No cross-reactivity is observed with IgG1, IgG2, IgG3, IgM, IgA (Jefferis et al. 1985).
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
0.05% ProClin 300, 0.002%Methylisothiazolone, 0.002% Bromonitrodioxane
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified IgG4
Target Isotype
IgG4 Fc
Purification
Protein A purified
Conjugation
Horseradish peroxidase(HRP)
RRID
AB_377048
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.