Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1/40-1/80 |
1/25,600-1/51,200 |
Assay dependent |
Not tested in other applications.
Product Note
This antibody recognizes the heavy chain of human IgG4, at an epitope in the Fc region. No cross-reactivity is observed with IgG1, IgG2, IgG3, IgM, IgA (Jefferis et al. 1985).
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
0.05% ProClin 300, 0.002%Methylisothiazolone, 0.002% Bromonitrodioxane
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified IgG4
Target Isotype
IgG4 Fc
Purification
Protein A purified
Conjugation
Horseradish peroxidase(HRP)
RRID
AB_377048
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.