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Mouse Asporin protein (active)

Mouse Asporin protein (active). GTX00331-pro
Mouse Asporin protein (active). GTX00331-pro
Mouse Asporin protein (active). GTX00331-pro

Cat No. GTX00331-pro

Application Functional Assay
Species Mouse
Package
10 μg ($289)
APPLICATION

Application Note

Asporin (ASPN) is a protein belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The protein negatively regulates chondrogenesis in the articular cartilage and periodontal ligament (PDL) differentiation, inhibits BMP2-induced cytodifferentiation of PDL cells and also nhibits the interaction between TGFB1 and TGF-beta receptor type II in the presence of heparin/heparan sulfate in vitro. Besides, Transforming Growth Factor Beta 2 (TGFb2) has been identified as an interactor of ASPN, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ASPN and recombinant mouse TGFb2. Briefly, ASPN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TGFb2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ASPN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of ASPN and TGFb2 was in a dose dependent manner.

Observed MW

71 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys16~Lys373 (NP_001165952.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

asporin , 4631401G09Rik , AA986886 , Plap1 , Slrr1c

Background

This gene encodes a member of the small leucine-rich proteoglycan family. The encoded protein is an extracellular matrix protein that modulates the transforming growth factor-beta signaling pathway, regulating cartilage matrix gene expression and cartilage formation. The protein plays a role in the pathology of osteoarthritis. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Database

DATA IMAGES
Mouse Asporin protein (active). GTX00331-pro

GTX00331-pro Functional Assay Image

Functional ELISA analysis of GTX00331-pro Mouse Asporin protein (active) which can bind immobilized TGFb2 protein.

Mouse Asporin protein (active). GTX00331-pro

GTX00331-pro Image

SDS-PAGE analysis of GTX00331-pro Mouse Asporin protein (active).

Mouse Asporin protein (active). GTX00331-pro

GTX00331-pro Image

WB analysis of GTX00331-pro Mouse Asporin protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289