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Mouse Asporin protein (active)

Mouse Asporin protein (active). GTX00331-pro
Mouse Asporin protein (active). GTX00331-pro
Mouse Asporin protein (active). GTX00331-pro

Cat No. GTX00331-pro

Application Functional Assay
Species Mouse
Package
10 μg ($289)
APPLICATION

Application Note

Asporin (ASPN) is a protein belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The protein negatively regulates chondrogenesis in the articular cartilage and periodontal ligament (PDL) differentiation, inhibits BMP2-induced cytodifferentiation of PDL cells and also nhibits the interaction between TGFB1 and TGF-beta receptor type II in the presence of heparin/heparan sulfate in vitro. Besides, Transforming Growth Factor Beta 2 (TGFb2) has been identified as an interactor of ASPN, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ASPN and recombinant mouse TGFb2. Briefly, ASPN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TGFb2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ASPN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of ASPN and TGFb2 was in a dose dependent manner.

Observed MW

71 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys16~Lys373 (NP_001165952.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

asporin,4631401G09Rik,AA986886,Plap1,Slrr1c

Background

This gene encodes a member of the small leucine-rich proteoglycan family. The encoded protein is an extracellular matrix protein that modulates the transforming growth factor-beta signaling pathway, regulating cartilage matrix gene expression and cartilage formation. The protein plays a role in the pathology of osteoarthritis. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Database

DATA IMAGES
Mouse Asporin protein (active). GTX00331-pro

GTX00331-pro Functional Assay Image

Functional ELISA analysis of GTX00331-pro Mouse Asporin protein (active) which can bind immobilized TGFb2 protein.

Mouse Asporin protein (active). GTX00331-pro

GTX00331-pro Image

SDS-PAGE analysis of GTX00331-pro Mouse Asporin protein (active).

Mouse Asporin protein (active). GTX00331-pro

GTX00331-pro Image

WB analysis of GTX00331-pro Mouse Asporin protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289