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Mouse BDNF protein (active)

Mouse BDNF protein (active). GTX00322-pro
Mouse BDNF protein (active). GTX00322-pro
Mouse BDNF protein (active). GTX00322-pro

Cat No. GTX00322-pro

Application Functional Assay
Species Mouse
Package
10 μg ($319)
APPLICATION

Application Note

Brain-derived neurotrophic factor, also known as BDNF, is a member of the neurotrophin family of growth factors, which are related to the canonical Nerve Growth Factor. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. Besides, Amyloid Precursor Protein (APP) has been identified as an interactor of BDNF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse BDNF and recombinant mouse APP. Briefly, BDNF were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to APP-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-BDNF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of BDNF and APP was in a dose dependent manner.

Observed MW

45 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Glu139~Arg249 (NP_001041604.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

brain derived neurotrophic factor

Background

The protein encoded by this gene is a member of the nerve growth factor family. It is involved in the growth, differentiation and survival of specific types of developing neurons both in the central nervous system (CNS) and the peripheral nervous system. It is also involved in regulating synaptic plasticity in the CNS. Expression of a similar gene in human is reduced in both Alzheimer's and Huntington disease patients. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES
Mouse BDNF protein (active). GTX00322-pro

GTX00322-pro Functional Assay Image

Functional ELISA analysis of GTX00322-pro Mouse BDNF protein (active) which can bind immobilized APP protein.

Mouse BDNF protein (active). GTX00322-pro

GTX00322-pro Image

WB analysis of GTX00322-pro Mouse BDNF protein (active).

Mouse BDNF protein (active). GTX00322-pro

GTX00322-pro Image

SDS-PAGE analysis of GTX00322-pro Mouse BDNF protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 319