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Mouse BMP7 protein, His tag (active)

Mouse BMP7 protein, His tag (active). GTX00296-pro
Mouse BMP7 protein, His tag (active). GTX00296-pro
Mouse BMP7 protein, His tag (active). GTX00296-pro
Mouse BMP7 protein, His tag (active). GTX00296-pro

Cat. No. GTX00296-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($309)
See all BMP7 protein products

APPLICATION

Application Note

BMP7 (Bone morphogenetic protein 7), which belongs to the TGF-β superfamily, is a signaling molecule with the ability to promote bone formation. BMP7 has also been implicated in various types of cancer, including breast cancer. It has been reported that BMP7 treatment induced cell growth promotion of MDA-MB-231 breast cancer line. To test the effect of BMP7 on cell proliferation,  MDA-MB-231 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of BMP7. After incubated for 72h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. The dose-effect curve that BMP7 significantly promoted cell proliferation of MDA-MB-231 cells. The ED₅₀ for this effect is typically 3. 483-9. 017 ng/ml.

Observed MW

17 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser292~His430 (NP_031583.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

bone morphogenetic protein 7 , OP1

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. Mutation of this gene results in skeletal, kidney, and other developmental defects. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Mouse BMP7 protein, His tag (active). GTX00296-pro

GTX00296-pro Functional Assay Image

Cell proliferation effect of GTX00296-pro Mouse BMP7 protein (active).
(A) Unstimulated MDA-MB-231 cells cultured in DMEM for 72hrs.
(B) MCF-7 cells cultured in DMEM, stimulated with 10 ng/ml BMP7 for 72hrs.

Mouse BMP7 protein, His tag (active). GTX00296-pro

GTX00296-pro Functional Assay Image

The dose effect curve of GTX00296-pro Mouse BMP7 protein (active) on MDA-MB-231 cells. After incubation of Mouse BMP7 protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Mouse BMP7 protein, His tag (active). GTX00296-pro

GTX00296-pro Image

WB analysis of GTX00296-pro Mouse BMP7 protein (active).

Mouse BMP7 protein, His tag (active). GTX00296-pro

GTX00296-pro Image

SDS-PAGE analysis of GTX00296-pro Mouse BMP7 protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 309