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Mouse COL6A1 protein, His tag

Mouse COL6A1 protein, His tag. GTX00316-pro
Mouse COL6A1 protein, His tag. GTX00316-pro
Mouse COL6A1 protein, His tag. GTX00316-pro

Cat. No. GTX00316-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($299)
See all COL6A1 protein products

APPLICATION

Application Note

Collagen alpha-1(VI) chain (COL6a1) is a protein that in humans, the collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Besides, Platelet-derived growth factor subunit A (PDGFA) has been identified as an interactor of COL6A1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human COL6a1 and recombinant human PDGFA. Briefly, COL6a1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PDGFA-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-COL6a1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of COL6a1 and PDGFA was in a dose dependent manner.

Observed MW

21 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser624~Lys800 (NP_034063.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

collagen, type VI, alpha 1 , AI747156 , Col6a-1

Database

Research Area

DATA IMAGES

Mouse COL6A1 protein, His tag. GTX00316-pro

GTX00316-pro Functional Assay Image

Functional ELISA analysis of GTX00316-pro Mouse COL6A1 protein which can bind immobilized PDGFA protein.

Mouse COL6A1 protein, His tag. GTX00316-pro

GTX00316-pro Image

SDS-PAGE analysis of GTX00316-pro Mouse COL6A1 protein.

Mouse COL6A1 protein, His tag. GTX00316-pro

GTX00316-pro Image

WB analysis of GTX00316-pro Mouse COL6A1 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 299