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Mouse CTLA4 protein, mutant mouse IgG2a Fc tag (active)

Cat. No. GTX65784-pro

Application

 Functional Assay

Species

 Mouse
Package
100 μg ($219)
See all CTLA4 protein products
PRODUCT

Summary

Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

APPLICATION

Application Note

Blocks the binding of mouse CD80 (B7-1) and CD86 (B7-2) to their receptors (by binding CD80 and CD86 with high affinity) and thereby prevents their T cell regulatory actions by inhibiting the CD28 signaling competitively. Shows the biological functions of the CD152 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain. Useful for investigating the T cell co-stimulation.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 0.2μm-filtered PBS to 100 μg/ml. Lyophilized from 0.2μm-filtered PBS.

Preservative

No preservative

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.

Region/Sequence

The extracellular domain of mouse CD152 [CTLA-4] (a.a. 1-160) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.

Expression System

NS-1 cells

Purity

??98% by SDS-PAGE.

Endotoxin

< 0.06 EU/μg

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytotoxic T-lymphocyte-associated protein 4 , Cd152 , Ctla-4 , Ly-56

Database

Research Area

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 219