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Mouse EGF protein, His tag (active)

Mouse EGF protein, His tag (active). GTX66978-pro

Cat. No. GTX66978-pro

Application

 Functional Assay

Species

 Mouse
Package
5 μg ($219)
See all EGF protein products

APPLICATION

Application Note

Measured in a cell proliferation assay using mouse Balb/3T3 cells. The ED₅₀ for this effect less or equal to 1 ng/ml.

PROPERTIES

Form

Liquid

Buffer

20mM Tris-HCl (pH8.0), 100mM NaCl, 2mM DTT, 10% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

N-terminal His-Tag; Length: 77 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR

Expression System

E. coli

Purity

> 95% by SDS-PAGE.

Endotoxin

< 0.2 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epidermal growth factor , AI790464

Background

This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Mouse EGF protein, His tag (active). GTX66978-pro

GTX66978-pro Image

3 μg of GTX66978-pro Mouse EGF protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

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SDS
Glycerol.pdf
Package List Price ($)
$ 219