GeneTex
  • Country / Location Selection

United States (US)

Mouse EGF protein (active)

Mouse EGF protein (active). GTX00327-pro
Mouse EGF protein (active). GTX00327-pro
Mouse EGF protein (active). GTX00327-pro

Cat No. GTX00327-pro

Application

Functional Assay

Species

Mouse
Package
10 μg ($229)

APPLICATION

Application Note

Epidermal growth factor (EGF) binding to EGFR, results in cellular proliferation, differentiation, and survival. To test the effect of EGF on cell proliferation, 3T3 fibroblasts cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 1% serum standard DMEM  prior to the addition of various concentrations of recombinant mouse EGF. After incubated for 96h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of 3T3 cells after incubation with EGF for 96h observed by inverted microscope.

Observed MW

38 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Asn977~Arg1029 (NP_034243.2)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epidermal growth factor , AI790464

Background

This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Mouse EGF protein (active). GTX00327-pro

GTX00327-pro Functional Assay Image

Cell proliferation effect of GTX00327-pro Mouse EGF protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated 3T3 cells cultured in DMEM for 96hrs.
(B) 3T3 cells cultured in DMEM, stimulated with 1 ng/ml EGF for 96hrs.

Mouse EGF protein (active). GTX00327-pro

GTX00327-pro Image

WB analysis of GTX00327-pro Mouse EGF protein (active).

Mouse EGF protein (active). GTX00327-pro

GTX00327-pro Image

SDS-PAGE analysis of GTX00327-pro Mouse EGF protein (active).

REFERENCE

There are currently no references for Mouse EGF protein (active) (GTX00327-pro). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Mouse EGF protein (active) (GTX00327-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 229