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Mouse FGF1 protein (active)

Mouse FGF1 protein (active). GTX00281-pro
Mouse FGF1 protein (active). GTX00281-pro
Mouse FGF1 protein (active). GTX00281-pro

Cat No. GTX00281-pro

Application Functional Assay
Species Mouse
Package
10 μg ($279)
APPLICATION

Application Note

FGF1 (Fibroblast growth factor 1) belongs to the fibroblast growth factor (FGF) family. FGF1 plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 is thought to stimulate the proliferation of 3T3 fibroblasts. Thus, a cell proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF1 using 3T3 fibroblasts. Briefly,  3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of FGF1. After incubated for 48h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of 3T3 cells after incubation with FGF1 for 48h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF1 for 48h. And FGF1 significantly increased cell viability of 3T3 cells.

Observed MW

18 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Phe16~Asp155 (NP_034327.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

fibroblast growth factor 1,Dffrx,Fam,Fgf-1,Fgfa

Database

Research Area

DATA IMAGES
Mouse FGF1 protein (active). GTX00281-pro

GTX00281-pro Functional Assay Image

Cell proliferation effect of GTX00281-pro Mouse FGF1 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated 3T3 cells cultured in DMEM for 48hrs.
(B) 3T3 cells cultured in DMEM, stimulated with 100 ng/ml FGF1 for 48hrs.

Mouse FGF1 protein (active). GTX00281-pro

GTX00281-pro Image

SDS-PAGE analysis of GTX00281-pro Mouse FGF1 protein (active).

Mouse FGF1 protein (active). GTX00281-pro

GTX00281-pro Image

WB analysis of GTX00281-pro Mouse FGF1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 279