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Mouse FGF2 protein (active)

Mouse FGF2 protein (active). GTX00310-pro
Mouse FGF2 protein (active). GTX00310-pro
Mouse FGF2 protein (active). GTX00310-pro

Cat No. GTX00310-pro

Application Functional Assay
Species Mouse
Package
10 μg ($279)
APPLICATION

Application Note

Basic fibroblast growth factor (FGF2), also known as bFGF, FGF-β is a member of a large family of structurally related heparin-binding proteins (the FGFs) involved in the regulation of cell proliferation, growth and differentiation. It involved in many biological processes including angiogenesis, embryonic development and wound healing. Additionally, FGF2 is a critical component of human embryonic stem cell culture medium. Besides, Caspase 1 (CASP1) has been identified as an interactor of FGF2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse FGF2 and recombinant mouse CASP1. Briefly, FGF2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CASP1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-FGF2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of FGF2 and CASP1 was in a dose dependent manner.

Observed MW

20/23 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro10~Ser154 (NP_032032.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Mouse FGF2 protein (active). GTX00310-pro

GTX00310-pro Functional Assay Image

Functional ELISA analysis of GTX00310-pro Mouse FGF2 protein (active) which can bind immobilized CASP1 protein.

Mouse FGF2 protein (active). GTX00310-pro

GTX00310-pro Image

SDS-PAGE analysis of GTX00310-pro Mouse FGF2 protein (active).

Mouse FGF2 protein (active). GTX00310-pro

GTX00310-pro Image

WB analysis of GTX00310-pro Mouse FGF2 protein (active).

REFERENCE
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REVIEW
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