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Mouse FGF2 protein (active)

Mouse FGF2 protein (active). GTX00310-pro
Mouse FGF2 protein (active). GTX00310-pro
Mouse FGF2 protein (active). GTX00310-pro

Cat No. GTX00310-pro

Application

Functional Assay

Species

Mouse
Package
10 μg ($279)

APPLICATION

Application Note

Basic fibroblast growth factor (FGF2), also known as bFGF, FGF-β is a member of a large family of structurally related heparin-binding proteins (the FGFs) involved in the regulation of cell proliferation, growth and differentiation. It involved in many biological processes including angiogenesis, embryonic development and wound healing. Additionally, FGF2 is a critical component of human embryonic stem cell culture medium. Besides, Caspase 1 (CASP1) has been identified as an interactor of FGF2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse FGF2 and recombinant mouse CASP1. Briefly, FGF2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CASP1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-FGF2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of FGF2 and CASP1 was in a dose dependent manner.

Observed MW

20/23 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro10~Ser154 (NP_032032.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibroblast growth factor 2 , Fgf-2 , Fgfb , bFGF

Database

Research Area

DATA IMAGES

Mouse FGF2 protein (active). GTX00310-pro

GTX00310-pro Functional Assay Image

Functional ELISA analysis of GTX00310-pro Mouse FGF2 protein (active) which can bind immobilized CASP1 protein.

Mouse FGF2 protein (active). GTX00310-pro

GTX00310-pro Image

SDS-PAGE analysis of GTX00310-pro Mouse FGF2 protein (active).

Mouse FGF2 protein (active). GTX00310-pro

GTX00310-pro Image

WB analysis of GTX00310-pro Mouse FGF2 protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 279