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Mouse FGF22 protein (active)

Mouse FGF22 protein (active). GTX00299-pro
Mouse FGF22 protein (active). GTX00299-pro
Mouse FGF22 protein (active). GTX00299-pro

Cat No. GTX00299-pro

Application Functional Assay
Species Mouse
Package
10 μg ($289)
APPLICATION

Application Note

FGF22 (Fibroblast growth factor 22) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF22 using 3T3 cells. Briefly,  3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of FGF22. After incubated for 48h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of 3T3 cells after incubation with FGF22 for 48h observed by inverted microscope Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF22 for 48h. And FGF22 significantly increased cell viability of 3T3 cells.

Observed MW

20 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; His26~Ser162 (NP_075793.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

fibroblast growth factor 22,2210414E06Rik,FGF-22

Database

DATA IMAGES
Mouse FGF22 protein (active). GTX00299-pro

GTX00299-pro Functional Assay Image

Cell proliferation effect of GTX00299-pro Mouse FGF22 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated 3T3 cells cultured in DMEM for 48hrs.
(B) 3T3 cells cultured in DMEM, stimulated with 1000 ng/ml FGF22 for 48hrs.

Mouse FGF22 protein (active). GTX00299-pro

GTX00299-pro Image

WB analysis of GTX00299-pro Mouse FGF22 protein (active).

Mouse FGF22 protein (active). GTX00299-pro

GTX00299-pro Image

SDS-PAGE analysis of GTX00299-pro Mouse FGF22 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289