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Mouse FGF23 protein, His tag (active)

Mouse FGF23 protein, His tag (active). GTX00295-pro
Mouse FGF23 protein, His tag (active). GTX00295-pro
Mouse FGF23 protein, His tag (active). GTX00295-pro

Cat. No. GTX00295-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($339)
See all FGF23 protein products

APPLICATION

Application Note

FGF23 (Fibroblast growth factor 23) is a member of the fibroblast growth factor family, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. A proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF23 using 3T3 cells. Briefly,  3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of FGF23. After incubated for 48h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of 3T3 cells after incubation with FGF23 for 48h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF23 for 48h. And FGF23 significantly increased cell viability of 3T3 cells.

Observed MW

29 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Tyr25~Val251 (NP_073148.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibroblast growth factor 23

Background

This gene encodes a member of the fibroblast growth factor family. The encoded protein regulates phosphate homeostasis and vitamin D metabolism. Mutation of the related gene in humans causes autosomal dominant hypophosphatemic rickets (ADHR). The secreted protein is further cleaved into N- and C-terminal chains, which results in loss of function. [provided by RefSeq, Mar 2013]

Database

Research Area

DATA IMAGES

Mouse FGF23 protein, His tag (active). GTX00295-pro

GTX00295-pro Functional Assay Image

Cell proliferation effect of GTX00295-pro Mouse FGF23 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated 3T3 cells cultured in DMEM for 48hrs.
(B) 3T3 cells cultured in DMEM, stimulated with 1000 ng/ml FGF23 for 48hrs.

Mouse FGF23 protein, His tag (active). GTX00295-pro

GTX00295-pro Image

SDS-PAGE analysis of GTX00295-pro Mouse FGF23 protein (active).

Mouse FGF23 protein, His tag (active). GTX00295-pro

GTX00295-pro Image

WB analysis of GTX00295-pro Mouse FGF23 protein (active).

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SDS
Proclin.pdf
Package List Price ($)
$ 339