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Mouse GDF8 / Myostatin protein (active)

Mouse GDF8 / Myostatin protein (active). GTX00308-pro
Mouse GDF8 / Myostatin protein (active). GTX00308-pro
Mouse GDF8 / Myostatin protein (active). GTX00308-pro

Cat No. GTX00308-pro

Application Functional Assay
Species Mouse
Package
10 μg ($249)
APPLICATION

Application Note

Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) a myokine, a protein produced and released by myocytes. It inhibit myogenesis including muscle cell growth and differentiation. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. Besides, Follistatin Like Protein 3 (FSTL3) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse MSTN and recombinant mouse FSTL3. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FSTL3-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of MSTN and FSTL3 was in a dose dependent manner.

Observed MW

15 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asp268~Ser376 (NP_034964.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

myostatin,Cmpt,Gdf8

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Homozygous knockout mice for this gene exhibit increased muscle mass and bone density, and reduced adiposity. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES
Mouse GDF8 / Myostatin protein (active). GTX00308-pro

GTX00308-pro Functional Assay Image

Functional ELISA analysis of GTX00308-pro Mouse GDF8 / Myostatin protein (active) which can bind immobilized FSTL3 protein.

Mouse GDF8 / Myostatin protein (active). GTX00308-pro

GTX00308-pro Image

WB analysis of GTX00308-pro Mouse GDF8 / Myostatin protein (active).

Mouse GDF8 / Myostatin protein (active). GTX00308-pro

GTX00308-pro Image

SDS-PAGE analysis of GTX00308-pro Mouse GDF8 / Myostatin protein (active).

REFERENCE
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Package List Price ($)
$ 249