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Mouse GOLPH2 protein, His and GST tag

Mouse GOLPH2 protein, His and GST tag. GTX00302-pro
Mouse GOLPH2 protein, His and GST tag. GTX00302-pro
Mouse GOLPH2 protein, His and GST tag. GTX00302-pro

Cat. No. GTX00302-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($359)
See all GOLPH2 protein products

APPLICATION

Application Note

Golgi Protein 73 (GP73) also known as Golgi phosphoprotein 2 or Golgi membrane protein GP73 is a protein that encoded by the GOLM1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. It has been observed to be upregulated in response to viral infection. Besides, Dymeclin (DYM) has been identified as an interactor of GP73, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse GP73 and recombinant human DYM. Briefly, GP73 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to DYM-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-GP73 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of GP73 and DYM was in a dose dependent manner.

Observed MW

58 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Ser133~Ser379 (NP_001030294.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

golgi membrane protein 1 , 2310001L02Rik , AW125446 , D030064E01Rik , GP73 , Golph2 , PSEC0257

Database

Research Area

DATA IMAGES

Mouse GOLPH2 protein, His and GST tag. GTX00302-pro

GTX00302-pro Functional Assay Image

Functional ELISA analysis of GTX00302-pro Mouse GOLPH2 protein which can bind immobilized DYM protein.

Mouse GOLPH2 protein, His and GST tag. GTX00302-pro

GTX00302-pro Image

WB analysis of GTX00302-pro Mouse GOLPH2 protein.

Mouse GOLPH2 protein, His and GST tag. GTX00302-pro

GTX00302-pro Image

SDS-PAGE analysis of GTX00302-pro Mouse GOLPH2 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 359