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Mouse Glypican 4 protein (active)

Mouse Glypican 4 protein (active). GTX00330-pro
Mouse Glypican 4 protein (active). GTX00330-pro
Mouse Glypican 4 protein (active). GTX00330-pro

Cat No. GTX00330-pro

Application Functional Assay
Species Mouse
Package
10 μg ($309)
APPLICATION

Application Note

Glypican 4 (GPC4) is a cell surface heparan sulfate proteoglycans which composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Besides, Fibroblast Growth Factor 2, Basic (FGF2) has been identified as an interactor of GPC4, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse GPC4 and recombinant mouse FGF2. Briefly, GPC4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FGF2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-GPC4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of GPC4 and FGF2 was in a dose dependent manner.

Observed MW

27 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Thr220~Gly426 (NP_032176.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glypican 4,9530073D23Rik,AF311610,AI385680,AI661372,K-glypican

Database

Research Area

DATA IMAGES
Mouse Glypican 4 protein (active). GTX00330-pro

GTX00330-pro Functional Assay Image

Functional ELISA analysis of GTX00330-pro Mouse Glypican 4 protein (active) which can bind immobilized FGF2 protein.

Mouse Glypican 4 protein (active). GTX00330-pro

GTX00330-pro Image

WB analysis of GTX00330-pro Mouse Glypican 4 protein (active).

Mouse Glypican 4 protein (active). GTX00330-pro

GTX00330-pro Image

SDS-PAGE analysis of GTX00330-pro Mouse Glypican 4 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 309