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Mouse Gremlin 1 protein (active)

Mouse Gremlin 1 protein (active). GTX00318-pro
Mouse Gremlin 1 protein (active). GTX00318-pro
Mouse Gremlin 1 protein (active). GTX00318-pro

Cat No. GTX00318-pro

Application Functional Assay
Species Mouse
Package
10 μg ($279)
APPLICATION

Application Note

Gremlin (GREM1) is an inhibitor in the TGF beta signaling pathway. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. A proliferation assay was conducted to detect the bioactivity of recombinant mouse GREM1 using A549 cells. Briefly,  A549 cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM  prior to the addition of various concentrations of GREM1. After incubated for 96h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of A549 cells after incubation with GREM1 for 96h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant GREM1 for 96h that GREM1 significantly increased cell viability of A549 cells.

Observed MW

27 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys25~Asp184 (NP_035954.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

gremlin 1, DAN family BMP antagonist , Cktsf1b1 , Drm , Grem , ld

Database

Research Area

DATA IMAGES
Mouse Gremlin 1 protein (active). GTX00318-pro

GTX00318-pro Functional Assay Image

Cell proliferation effect of GTX00318-pro Mouse Gremlin 1 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated A549 cells cultured in DMEM for 96hrs.
(B) A549 cells cultured in DMEM, stimulated with 1 ng/ml GREM1 for 96hrs.

Mouse Gremlin 1 protein (active). GTX00318-pro

GTX00318-pro Image

WB analysis of GTX00318-pro Mouse Gremlin 1 protein (active).

Mouse Gremlin 1 protein (active). GTX00318-pro

GTX00318-pro Image

SDS-PAGE analysis of GTX00318-pro Mouse Gremlin 1 protein (active).

REFERENCE
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Package List Price ($)
$ 279