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Mouse IL1 Receptor antagonist protein, His tag

Mouse IL1 Receptor antagonist protein, His tag. GTX00289-pro
Mouse IL1 Receptor antagonist protein, His tag. GTX00289-pro
Mouse IL1 Receptor antagonist protein, His tag. GTX00289-pro

Cat. No. GTX00289-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($309)
See all IL1 Receptor antagonist protein products

APPLICATION

Application Note

IL1RA (interleukin-1 receptor antagonist) is an agent that binds to the cell surface interleukin-1 receptor (IL-1R), which would prevent IL-1 from intracellular signal transduction. Besides, mouse IL1R1 and rat IL1R1 share similarities in amino acid sequence with the identity of 85.6% . Thus, a binding ELISA assay was constructed to detect the association of recombinant mouse IL1RA with recombinant rat IL1R1. Briefly, IL1RA were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL1R1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL1RA with IL1R1 was in a dose dependent manner.

Observed MW

20 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Arg27~Gln178 (NP_001034790.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 receptor antagonist , F630041P17Rik , IL-1ra

Database

Research Area

DATA IMAGES

Mouse IL1 Receptor antagonist protein, His tag. GTX00289-pro

GTX00289-pro Functional Assay Image

Functional ELISA analysis of GTX00289-pro Mouse IL1 Receptor antagonist protein which can bind immobilized IL1R1 protein.

Mouse IL1 Receptor antagonist protein, His tag. GTX00289-pro

GTX00289-pro Image

SDS-PAGE analysis of GTX00289-pro Mouse IL1 Receptor antagonist protein.

Mouse IL1 Receptor antagonist protein, His tag. GTX00289-pro

GTX00289-pro Image

WB analysis of GTX00289-pro Mouse IL1 Receptor antagonist protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 309