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Mouse IL1 beta protein, His tag (active)

Mouse IL1 beta protein, His tag (active). GTX00293-pro
Mouse IL1 beta protein, His tag (active). GTX00293-pro
Mouse IL1 beta protein, His tag (active). GTX00293-pro

Cat. No. GTX00293-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($359)
See all IL1 beta protein products

APPLICATION

Application Note

IL-1β (Interleukin-1 beta) is a proinflammatory and immunoregulatory cytokine involved in a variety of cellular activities. It has been elucidated that IL-1β stimulation of cells activates MMP-9 (matrix metalloproteinases-9) secretion by the activation of the dual signalling pathways. Thus, a stimulation assay of IL-1β was conducted using 3T3 cell line, and the MMP-9 activity was detected through gel zymography. Briefly, 1×1x10⁶ 3T3 cells were cultured overnight in 5% DMEM, washed with serum-free medium and then stimulated with different concentrations of IL-1β for 20h, and cell lysates were collected to measure MMP-9 activity. Cell lysates samples were denatured by SDS loading buffer, electrophoresed through sodium dodecyl sμl phate–polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and coomassiebrilliant blue (CBB)-stained, MMPs hydrolyzed gelatin nearby, indicated by the white binds on the gel. Increased MMP-9 activity in 3T3 cells due to the stimulation of IL-1β.

Observed MW

19 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Val118~Ser269 (NP_032387.1)

Expression System

HEK293 cells

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 beta , IL-1beta , Il-1b

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Mouse IL1 beta protein, His tag (active). GTX00293-pro

GTX00293-pro Functional Assay Image

ELISA detection of secerted MMP9 protein activation from 3T3 cells induced by GTX00293-pro Mouse IL1 beta protein (active) for 20hrs.
Recombinant protein concentrations: 0.5 and 1 ng/ml.

Mouse IL1 beta protein, His tag (active). GTX00293-pro

GTX00293-pro Image

WB analysis of GTX00293-pro Mouse IL1 beta protein (active).

Mouse IL1 beta protein, His tag (active). GTX00293-pro

GTX00293-pro Image

SDS-PAGE analysis of GTX00293-pro Mouse IL1 beta protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 359