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Mouse IL12 protein (active)

Cat No. GTX53872-pro

Application

Functional Assay

Species

Mouse
Package
10 μg ($199)
PRODUCT

Summary

Recombinant murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues). Verified by N-terminal and Mass Spectrometry analyses (when applicable).

APPLICATION

Application Note

Assay #1: Determined by its ability to induce cell proliferation in T-cell enriched PBMCs. The expected ED₅₀ is ≤0.1ng/ml, corresponding to a specific activity of ≥1 x 10⁷units/mg.
Assay #2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED₅₀ is ≤0.1ng/ml, corresponding to a specific activity of ≥1 x 10⁷units/mg.

Specificity/Sensitivity

cross reactivity: Human

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.

Region/Sequence

p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA
p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS

Expression System

CHO cells

Purity

≥ 98% by SDS-PAGE and HPLC.

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 12a , IL-12p35 , Il-12a , Ll12a , p35

Database

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 199