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Mouse IL2 Receptor alpha protein (active)

Mouse IL2 Receptor alpha protein (active). GTX00298-pro
Mouse IL2 Receptor alpha protein (active). GTX00298-pro
Mouse IL2 Receptor alpha protein (active). GTX00298-pro

Cat No. GTX00298-pro

Application

Functional Assay

Species

Mouse
Package
10 μg ($249)

APPLICATION

Application Note

IL2RA (Interleukin-2 receptor alpha chain ), also known as Tac antigen and as CD25, was initially identified as a 55kDa membrane glycoprotein which is capable of binding IL 2. Besides, mouse IL2 shares 75.1% AA sequence identity with rat IL2, suggesting the exist of cross-species activity. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant rat IL2RA with recombinant mouse IL2. Briefly, IL2RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL2RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL2RA with IL2 was in a dose dependent manner.

Observed MW

37 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Glu22~Lys236 (NP_032393.3)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Mouse IL2 Receptor alpha protein (active). GTX00298-pro

GTX00298-pro Functional Assay Image

Functional ELISA analysis of GTX00298-pro Mouse IL2 Receptor alpha protein (active) which can bind immobilized IL2 protein.

Mouse IL2 Receptor alpha protein (active). GTX00298-pro

GTX00298-pro Image

SDS-PAGE analysis of GTX00298-pro Mouse IL2 Receptor alpha protein (active).

Mouse IL2 Receptor alpha protein (active). GTX00298-pro

GTX00298-pro Image

WB analysis of GTX00298-pro Mouse IL2 Receptor alpha protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 249