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Mouse IL2 Receptor alpha protein (active)

Mouse IL2 Receptor alpha protein (active). GTX00298-pro
Mouse IL2 Receptor alpha protein (active). GTX00298-pro
Mouse IL2 Receptor alpha protein (active). GTX00298-pro

Cat No. GTX00298-pro

Application

Functional Assay

Species

Mouse
Package
10 μg ($249)

APPLICATION

Application Note

IL2RA (Interleukin-2 receptor alpha chain ), also known as Tac antigen and as CD25, was initially identified as a 55kDa membrane glycoprotein which is capable of binding IL 2. Besides, mouse IL2 shares 75.1% AA sequence identity with rat IL2, suggesting the exist of cross-species activity. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant rat IL2RA with recombinant mouse IL2. Briefly, IL2RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL2RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL2RA with IL2 was in a dose dependent manner.

Observed MW

37 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Glu22~Lys236 (NP_032393.3)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 2 receptor, alpha chain , CD25 , Il2r , Ly-43

Database

Research Area

DATA IMAGES

Mouse IL2 Receptor alpha protein (active). GTX00298-pro

GTX00298-pro Functional Assay Image

Functional ELISA analysis of GTX00298-pro Mouse IL2 Receptor alpha protein (active) which can bind immobilized IL2 protein.

Mouse IL2 Receptor alpha protein (active). GTX00298-pro

GTX00298-pro Image

SDS-PAGE analysis of GTX00298-pro Mouse IL2 Receptor alpha protein (active).

Mouse IL2 Receptor alpha protein (active). GTX00298-pro

GTX00298-pro Image

WB analysis of GTX00298-pro Mouse IL2 Receptor alpha protein (active).

REFERENCE

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