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Mouse IL2 Receptor beta protein, His tag

Mouse IL2 Receptor beta protein, His tag. GTX00290-pro
Mouse IL2 Receptor beta protein, His tag. GTX00290-pro
Mouse IL2 Receptor beta protein, His tag. GTX00290-pro

Cat. No. GTX00290-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($309)
See all IL2 Receptor beta protein products

APPLICATION

Application Note

IL2RB (interleukin-2 receptor subunit beta) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, which binds and responds to a cytokine called IL-2. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant mouse IL2RB with recombinant mouse IL2. Briefly, IL2RB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL2RB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL2RB with IL2 was in a dose dependent manner.

Observed MW

27 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala27~Glu240 (NP_032394.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 2 receptor, beta chain , CD122 , IL-15Rbeta , IL15Rbeta , Il-2/15Rbeta , Il-2Rbeta , p70

Background

The interleukin 2 receptor is composed of alpha and beta subunits. The beta subunit encoded by this gene is very homologous to the human beta subunit and also shows structural similarity to other cytokine receptors. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Mouse IL2 Receptor beta protein, His tag. GTX00290-pro

GTX00290-pro Functional Assay Image

Functional ELISA analysis of GTX00290-pro Mouse IL2 Receptor beta protein which can bind immobilized IL2 protein.

Mouse IL2 Receptor beta protein, His tag. GTX00290-pro

GTX00290-pro Image

WB analysis of GTX00290-pro Mouse IL2 Receptor beta protein.

Mouse IL2 Receptor beta protein, His tag. GTX00290-pro

GTX00290-pro Image

SDS-PAGE analysis of GTX00290-pro Mouse IL2 Receptor beta protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 309