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Mouse IL2 protein, His tag (active)

Mouse IL2 protein, His tag (active). GTX00285-pro
Mouse IL2 protein, His tag (active). GTX00285-pro
Mouse IL2 protein, His tag (active). GTX00285-pro
Mouse IL2 protein, His tag (active). GTX00285-pro

Cat No. GTX00285-pro

Application

Functional Assay

Species

Mouse
Package
10 μg ($249)

APPLICATION

Application Note

Interleukin-2   (IL-2), the main lymphokine produced by activated CD4 T-helper lymphocytes, is a potent T-cell growth regulator. In addition to this activity, IL-2 was found to decrease the proliferation of 3T3 fibroblasts in time- and dose-dependent manner. Thus 3T3 cells were seeded overnight at a density of 5000 cells/well, and treated with or without various concentrations of IL2 for 48h, then 3T3 cells were observed by inverted microscope. The inhibitory effect of IL-2 on cell proliferation of 3T3 cells observed by inverted microscope. IL2 significantly decreased cell growth of 3T3 cells, and the ED₅₀ for this effect is typically 9. 876-13. 35 ng/ml.

Observed MW

25 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala21~Gln169 (NP_032392.1)

Expression System

HEK293 cells

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 2 , Il-2

Database

Research Area

DATA IMAGES

Mouse IL2 protein, His tag (active). GTX00285-pro

GTX00285-pro Functional Assay Image

The dose effect curve of GTX00285-pro Mouse IL2 protein (active) on 3T3 cells. After incubation of Mouse IL2 protein (active) for 48 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Mouse IL2 protein, His tag (active). GTX00285-pro

GTX00285-pro Functional Assay Image

The inhibition effect of GTX00285-pro Mouse IL2 protein (active) on cell proliferation.
(A) Unstimulated 3T3 cells cultured in DMEM for 72hrs.
(B) 3T3 cells cultured in DMEM, stimulated with IL2 (10 ng/ml) for 72hrs.

Mouse IL2 protein, His tag (active). GTX00285-pro

GTX00285-pro Image

SDS-PAGE analysis of GTX00285-pro Mouse IL2 protein (active).

Mouse IL2 protein, His tag (active). GTX00285-pro

GTX00285-pro Image

WB analysis of GTX00285-pro Mouse IL2 protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 249