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Mouse IL4 protein (active)

Mouse IL4 protein (active). GTX00329-pro
Mouse IL4 protein (active). GTX00329-pro
Mouse IL4 protein (active). GTX00329-pro

Cat No. GTX00329-pro

Application Functional Assay
Species Mouse
Package
10 μg ($249)
APPLICATION

Application Note

The interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL4, Th2 cells subsequently produce additional IL4 in a positive feedback loop. IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE, and up-regulates MHC class II production. IL4 decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL12. Besides, Interleukin 2 Receptor Gamma (IL2Rg) has been identified as an interactor of IL4, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IL4 and recombinant mouse IL2Rg. Briefly, IL4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL2Rg-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL4 and IL2Rg was in a dose dependent manner.

Observed MW

44 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; His21~Ser140 (NP_067258.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Mouse IL4 protein (active). GTX00329-pro

GTX00329-pro Functional Assay Image

Functional ELISA analysis of GTX00329-pro Mouse IL4 protein (active) which can bind immobilized IL2Rg protein.

Mouse IL4 protein (active). GTX00329-pro

GTX00329-pro Image

SDS-PAGE analysis of GTX00329-pro Mouse IL4 protein (active).

Mouse IL4 protein (active). GTX00329-pro

GTX00329-pro Image

WB analysis of GTX00329-pro Mouse IL4 protein (active).

REFERENCE
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REVIEW
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