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Mouse IL6 Receptor protein, His tag

Mouse IL6 Receptor protein, His tag. GTX00297-pro
Mouse IL6 Receptor protein, His tag. GTX00297-pro
Mouse IL6 Receptor protein, His tag. GTX00297-pro

Cat. No. GTX00297-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($249)
See all IL6 Receptor protein products

APPLICATION

Application Note

IL6R (Interleukin-6 receptor subunit alpha) is part of the receptor for interleukin 6. The interaction of IL6R and IL6 may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Thus, a binding ELISA assay was conducted to detect the association of IL6R with IL6. Briefly, IL6R were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL6-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL6R pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL6R with IL6 was in a dose dependent manner.

Observed MW

18 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.4), 0.01% SKL, 1mM DTT, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala52~Ala204 (NP_034689.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 6 receptor, alpha , CD126 , IL-6R , IL-6R-alpha , IL-6RA , Il6r

Database

Research Area

DATA IMAGES

Mouse IL6 Receptor protein, His tag. GTX00297-pro

GTX00297-pro Functional Assay Image

Functional ELISA analysis of GTX00297-pro Mouse IL6 Receptor protein which can bind immobilized IL6 protein.

Mouse IL6 Receptor protein, His tag. GTX00297-pro

GTX00297-pro Image

SDS-PAGE analysis of GTX00297-pro Mouse IL6 Receptor protein.

Mouse IL6 Receptor protein, His tag. GTX00297-pro

GTX00297-pro Image

WB analysis of GTX00297-pro Mouse IL6 Receptor protein.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Proclin.pdf
Package List Price ($)
$ 249